Mătase fluorescentă de culoare roșie poate ucide bacteriile dăunătoare ca remediu biologic și ecologic(Far-red fluorescent silk can kill harmful bacteria as biomedical and environmental remedy)

Un material hibrid de mătase atacă bacteriile atunci când este iluminat de o lumină verde, datorită cercetătorilor de proteină fluorescenți de departe roșii transferați la machiajul lor genetic.Materialul complet natural ar fi mai sigur decât mijloacele convenționale fotocatalitic sau activat cu lumină pentru a ucide agenții patogeni daunatori, cum ar fi bacteriile, care utilizează semiconductori potențial biohazard și necesită lumină ultravioletă care provoacă cancer pentru activare.Tehnologia de procesare a mătăsii fluorescente (mKate2) poate fi aplicată dezvoltării de bandaje inteligente pentru vindecarea rănilor pe pielea umană
O alternativă de mătase proiectată de Universitatea Purdue și Institutul Național de Cercetări Agricole coreene ar folosi biomateriale plasmonice asemănătoare biomaterialelor și lumină vizibilă, care, de asemenea, ajută la vindecarea rănilor și la reabilitarea mediului, inclusiv purificarea aerului și a apei.

Posted: Apr 19, 2018

Far-red fluorescent silk can kill harmful bacteria as biomedical and environmental remedy

(Nanowerk News) A silk hybrid material attacks bacteria when illuminated by a green light, thanks to a far-red fluorescent protein researchers transferred to its genetic makeup.
The all-natural material would be safer than conventional photocatalytic, or light-activated, means to kill harmful pathogens such as bacteria, which use potentially biohazardous semiconductors and require cancer-causing ultraviolet light for activation.
A silk alternative engineered by Purdue University and the Korean National Institute of Agricultural Research would instead use plasmonic photocatalyst-like biomaterials and visible light, which also aid in wound healing and environmental remediation including air and water purification. Their findings published in Advanced Science („Green-light-activated photoreaction via genetic hybridization of far-red fluorescent protein and silk”).
Silk Hybrid Bandage
The processing technology of fluorescent (mKate2) silk can be applied to developing smart medical bandages for wound healing on the human skin. (Image: Jung Woo Leem, Purdue University)
„Silk is an ancient and well-known biomaterial,” said Young Kim, Purdue associate professor of biomedical engineering. „It doesn’t have any issues with the human body. And the nice thing about green light is that it’s not harmful – the color corresponds to the strongest intensity of the solar spectrum.”
To combine the benefits of silk and green light, researchers inserted the gene for „mKate2,” a far-red fluorescent protein, into a silk host. Shining a green light on the resulting hybrid generates reactive oxygen species (ROS), which are effective radicals for breaking down organic contaminants and attacking the membrane and DNA of pathogens.
When E. coli on the fluorescent silk were illuminated by a weak green light for 60 minutes, the bacteria’s survival rate dropped to 45 percent.
The researchers found that the hybrid could be processed into a solution, film, bandage and fabric. „We’ve basically added fluorescence to silk to facilitate disinfection or decontamination using just visible light,” said Jung Woo Leem, a visiting scholar in Purdue’s school of biomedical engineering.
Kim’s team believes that green-light activated red fluorescent silk could be both more efficient and scalable than other plasmonic photocatalysts, in which metal nanoparticles hybridized from semiconductor materials also use visible light but could still pose negative environmental consequences.
„The silk photocatalysts would be easier and safer to produce than plasmonic ones since silkworms, rather than industrial facilities, provide the host for ROS-generating materials. It’s a completely new green manufacturing of nanomaterials,” Kim said.
Because ambient white light also includes green light, the researchers anticipate that the silk hybrid material should typically have a strong enough light source to generate ROS as long as a green light controls ROS generation.
Kim’s team plans to take advantage of silk’s biocompatibility with the human body both inside and out. „We’re thinking about some implantable and injectable wound healing materials that dissolve over time in the body. Then we wouldn’t need to do additional surgery to take it out,” Kim said.
Source: Purdue University